Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5.

ABSTRACT: Heme's spin-multiplicity is key in determining the enzymatic function of cytochrome P450 (cytP450). The origin of the low-spin state in ferric P450 is still under debate. Here, we report the first experimental demonstration of P450's membrane interaction altering its spin equilibrium which is accompanied by a stronger affinity for cytochrome b5. These results highlight the importance of lipid membrane for the function of P450.

SUBMITTER: Ravula T 

PROVIDER: S-EPMC5710748 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature