Jolkinolide B induces apoptosis of colorectal carcinoma through ROS-ER stress-Ca²⁺-mitochondria dependent pathway.

ABSTRACT: Colorectal carcinoma (CRC) remains one of the leading causes of death in cancer-related diseases. In this study, we aimed to investigate the anticancer effect of Jolkinolide B (JB), a bioactive diterpenoid component isolated from the dried roots of Euphorbia fischeriana Steud, on CRC cells and its underlying mechanisms. We found that JB suppressed the cell viability and colony formation of CRC cells, HT29 and SW620. Annexin V/PI assay revealed that JB induced apoptosis in CRC cells, which was further confirmed by the increased expression of cleaved-caspase3 and cleaved-PARP. iTRAQ-based quantitative proteomics was performed to identify JB-regulated proteins in CRC cells. Gene Ontology (GO) analysis revealed that these JB-regulated proteins were mainly involved in ER stress response,

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5710918 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature