Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.

ABSTRACT: Phosphatidylinositol-4,5-bisphosphate [PI(4,5)P₂] is essential for exocytosis. Classical ways of manipulating PI(4,5)P₂ levels are slower than its metabolism, making it difficult to distinguish effects of PI(4,5)P₂ from those of its metabolites. We developed a membrane-permeant, photoactivatable PI(4,5)P₂, which is loaded into cells in an inactive form and activated by light, allowing sub-second increases in PI(4,5)P₂ levels. By combining this compound with electrophysiological measurements in mouse adrenal chromaffin cells, we show that PI(4,5)P₂ uncaging potentiates exocytosis and identify synaptotagmin-1 (the Ca²⁺ sensor for exocytosis) and Munc13-2 (a vesicle priming protein) as the relevant effector proteins.

SUBMITTER: Walter AM 

PROVIDER: S-EPMC5711374 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature