A class-II myosin is required for growth, conidiation, cell wall integrity and pathogenicity of Magnaporthe oryzae.

ABSTRACT: In eukaryotic organisms, myosin proteins are the major ring components that are involved in cytokinesis. To date, little is known about the biologic functions of myosin proteins in Magnaporthe oryzae. In this study, insertional mutagenesis conducted in M. oryzae led to identification of Momyo2, a pathogenicity gene predicted to encode a class-II myosin protein homologous to Saccharomyces cerevisiae Myo1. According to qRT-PCR, Momyo2 is highly expressed during early infectious stage. When this gene was disrupted, the resultant mutant isolates were attenuated in virulence on rice and barley. These were likely caused by defective mycelial growth and frequent emergence of branch hyphae and septum. The Momyo2 mutants were also defective in conidial and appressorial development, characterized by

SUBMITTER: Guo M 

PROVIDER: S-EPMC5711394 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature