Persistence of Coxsackievirus B4 in pancreatic ductal-like cells results in cellular and viral changes.

ABSTRACT:

Introduction

Although known as cytolytic viruses, group B coxackieviruses (CVB) are able to establish a persistent infection in vitro and in vivo. Viral persistence has been reported as a key mechanism in the pathogenesis of CVB-associated chronic diseases such as type 1 diabetes (T1D). The impact of CVB4 persistence on human pancreas ductal-like cells was investigated.

Methods

A persistent CVB4 infection was established in ductal-like cells. PDX-1 expression, resistance to CVB4-induced lysis and CAR expression were evaluated. The profile of cellular microRNAs (miRNAs) was investigated through miRNA-sequencing. Viral phenotypic changes were examined, and genomic modifications were assessed by sequencing of the viral genome.

Results

The CVB4 persistence in ductal-like

SUBMITTER: Alidjinou EK 

PROVIDER: S-EPMC5711397 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature