Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection.

ABSTRACT: Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MS^E. In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins. Integration of transcriptional and proteomic data indicated that early in infection (6 h), P. brasiliensis yeast cells underwent a shift in metabolism from glycolysis to β-oxidation, upregulated detoxifying enzymes to defend against oxidative stress, and repres

SUBMITTER: Lacerda Pigosso L 

PROVIDER: S-EPMC5711425 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature