Retention and effective diffusion of model metabolites on porous graphitic carbon.

ABSTRACT: The study of metabolites in biological samples is of high interest for a wide range of biological and pharmaceutical applications. Reversed phase liquid chromatography is a common technique used for the separation of metabolites, but it provides little retention for polar metabolites. An alternative to C18 bonded phases, porous graphitic carbon has the ability to provide significant retention for both non-polar and polar analytes. The goal of this work is to study the retention and effective diffusion properties of porous graphitic carbon, to see if it is suitable for the wide injection bands and long run times associated with long, packed capillary-scale separations. The retention of a set of standard metabolites was studied for both stationary phases over a wide range of mobile phase con

SUBMITTER: Lunn DB 

PROVIDER: S-EPMC5711574 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature