The Impact of Phosphorylation on Electron Capture Dissociation of Proteins: A Top-Down Perspective.

ABSTRACT: Electron capture dissociation (ECD) is well suited for the characterization of phosphoproteins, with which labile phosphate groups are generally preserved during the fragmentation process. However, the impact of phosphorylation on ECD fragmentation of intact proteins remains unclear. Here, we have performed a systematic investigation of the phosphorylation effect on ECD of intact proteins by comparing the ECD cleavages of mono-phosphorylated α-casein, multi-phosphorylated β-casein, and immunoaffinity-purified phosphorylated cardiac troponin I with those of their unphosphorylated counterparts, respectively. In contrast to phosphopeptides, phosphorylation has significantly reduced deleterious effects on the fragmentation of intact proteins during ECD. On a global scale, the fragmentation pat

SUBMITTER: Chen B 

PROVIDER: S-EPMC5711594 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature