Differential gene expression elicited by children in response to the 2015-16 live attenuated versus inactivated influenza vaccine.

ABSTRACT:

Background

In recent influenza seasons, the live attenuated influenza vaccine (LAIV) has not demonstrated the same level of vaccine effectiveness as that observed among children who received the inactivated influenza vaccine (IIV). To better understand this difference, this study compared the mRNA sequencing transcription profile (RNA seq) in children who received either IIV or LAIV.

Methods

Children 3-17years of age receiving quadrivalent influenza vaccine were enrolled. Blood samples were collected on Day 0 prior to vaccination and again on Day 7 (range 6-10days) following vaccination. Total RNA was isolated from PAXgene tubes and sequenced for a custom panel of 89 transcripts using the TruSeq Targeted RNA Expression method. Fold differences in normalized RNA seq counts fr

SUBMITTER: Cole KS 

PROVIDER: S-EPMC5711596 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature