Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies.

ABSTRACT: Shigella spp. and Escherichia coli are closely related; both belong to the family Enterobacteriaceae. Phenotypically, Shigella spp. and E. coli share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant E. coli from Shigella spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism. Among these, identification using single nucleotide polymorphism is easy to perform and analyse, and it thus appears more promising. Among the nonmolecular methods, matrix-assisted desorption ionization-time

SUBMITTER: Devanga Ragupathi NK 

PROVIDER: S-EPMC5711669 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature