Development of a plasmid free CRISPR-Cas9 system for the genetic modification of Mucor circinelloides.

ABSTRACT: Mucor circinelloides and other members of Mucorales are filamentous fungi, widely used as model organisms in basic and applied studies. Although genetic manipulation methods have been described for some Mucoral fungi, construction of stable integrative transformants by homologous recombination has remained a great challenge in these organisms. In the present study, a plasmid free CRISPR-Cas9 system was firstly developed for the genetic modification of a Mucoral fungus. The described method offers a rapid but robust tool to obtain mitotically stable mutants of M. circinelloides via targeted integration of the desired DNA. It does not require plasmid construction and its expression in the recipient organism. Instead, it involves the direct introduction of the guide RNA and the Cas9 enzyme an

SUBMITTER: Nagy G 

PROVIDER: S-EPMC5711797 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature