Repurposing a photosynthetic antenna protein as a super-resolution microscopy label.

ABSTRACT: Techniques such as Stochastic Optical Reconstruction Microscopy (STORM) and Structured Illumination Microscopy (SIM) have increased the achievable resolution of optical imaging, but few fluorescent proteins are suitable for super-resolution microscopy, particularly in the far-red and near-infrared emission range. Here we demonstrate the applicability of CpcA, a subunit of the photosynthetic antenna complex in cyanobacteria, for STORM and SIM imaging. The periodicity and width of fabricated nanoarrays of CpcA, with a covalently attached phycoerythrobilin (PEB) or phycocyanobilin (PCB) chromophore, matched the lines in reconstructed STORM images. SIM and STORM reconstructions of Escherichia coli cells harbouring CpcA-labelled cytochrome bd ₁ ubiquinol oxidase in the cytoplasmic me

SUBMITTER: Barnett SFH 

PROVIDER: S-EPMC5711914 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature