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A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body.

ABSTRACT: Bacteria swim in sequences of straight runs that are interrupted by turning events. They drive their swimming locomotion with the help of rotating helical flagella. Depending on the number of flagella and their arrangement across the cell body, different run-and-turn patterns can be observed. Here, we present fluorescence microscopy recordings showing that cells of the soil bacterium Pseudomonas putida that are decorated with a polar tuft of helical flagella, can alternate between two distinct swimming patterns. On the one hand, they can undergo a classical push-pull-push cycle that is well known from monopolarly flagellated bacteria but has not been reported for species with a polar bundle of multiple flagella. Alternatively, upon leaving the pulling mode, they can enter a third slow swimming phase, where they propel themselves with their helical bundle wrapped around the cell body. A theoretical estimate based on a random-walk model shows that the spreading of a population of swimmers is strongly enhanced when cycling through a sequence of pushing, pulling, and wrapped flagellar configurations as compared to the simple push-pull-push pattern.

SUBMITTER: Hintsche M

PROVIDER: S-EPMC5711944 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body.

Hintsche Marius M Waljor Veronika V Großmann Robert R Kühn Marco J MJ Thormann Kai M KM Peruani Fernando F Beta Carsten C

Scientific reports 20171201 1

Bacteria swim in sequences of straight runs that are interrupted by turning events. They drive their swimming locomotion with the help of rotating helical flagella. Depending on the number of flagella and their arrangement across the cell body, different run-and-turn patterns can be observed. Here, we present fluorescence microscopy recordings showing that cells of the soil bacterium Pseudomonas putida that are decorated with a polar tuft of helical flagella, can alternate between two distinct s ...[more]

PMID: 29196650

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Project description:BackgroundThe block phase in the swimming start requires a quick reaction to the starting signal and a large take-off velocity that is primarily horizontal in direction. Due to the principle of specificity of training, there is a potential benefit of performing a greater proportion of horizontal force production exercises in a swimmers' dry-land resistance training sessions. Therefore, the purpose of this pilot study was to provide an insight into the effects of a horizontal- (HF) vs vertical-force (VF) training intervention on swim start performance.MethodsEleven competitive swimmers (six males (age 20.9 ± 1.8 years, body mass 77.3 ± 9.7 kg, height 1.78 ± 0.05 m) and five females (age 21.4 ± 2.0 years, body mass 67.5 ± 7.4 kg, height 1.69 ± 0.05 m)) completed 2 weekly sessions of either a horizontal- or vertical-force focused resistance training programme for 8 weeks. Squat jump force-time characteristics and swim start kinetic and kinematic parameters were collected pre- and post-intervention.ResultsAcross the study duration, the swimmers completed an average of nine swimming sessions per week with an average weekly swim volume of 45.5 ± 17.7 km (HF group) and 53 ± 20.0 km (VF group), but little practice of the swim start per week (n = 9). Within-group analyses indicated a significant increase in predicted one repetition maximum (1RM) hip thrust strength in the HF group, as well as significant increases in grab resultant peak force but reductions in resultant peak force of the block phase for the VF group. No significant between-group differences in predicted 1RM hip thrust and back squat strength, squat jump force-time and swim start performance measures were observed after 8 weeks of training. Significant correlations in the change scores of five block kinetic variables to time to 5 m were observed, whereby increased block kinetic outputs were associated with a reduced time to 5 m. This may be indicative of individual responses to the different training programmes.DiscussionThe results of this current study have been unable to determine whether a horizontal- or vertical-force training programme enhances swim start performance after an 8-week training intervention. Some reasons for the lack of within and between group effects may reflect the large volume of concurrent training and the relative lack of any deliberate practice of the swim start. Larger samples and longer training duration may be required to determine whether significant differences occur between these training approaches. Such research should also look to investigate how a reduction in the concurrent training loads and/or an increase in the deliberate practice of the swim start may influence the potential changes in swim start performance.

Dataset Information

A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body.

Publications

A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body.

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