Systematic identification of novel regulatory interactions controlling biofilm formation in the bacterium Escherichia coli.

ABSTRACT: Here, we investigated novel interactions of three global regulators of the network that controls biofilm formation in the model bacterium Escherichia coli using computational network analysis, an in vivo reporter assay and physiological validation experiments. We were able to map critical nodes that govern planktonic to biofilm transition and identify 8 new regulatory interactions for CRP, IHF or Fis responsible for the control of the promoters of rpoS, rpoE, flhD, fliA, csgD and yeaJ. Additionally, an in vivo promoter reporter assay and motility analysis revealed a key role for IHF as a repressor of cell motility through the control of FliA sigma factor expression. This investigation of first stage and mature biofilm formation indicates that biofilm structure is strongly affected by IHF a

SUBMITTER: Amores GR 

PROVIDER: S-EPMC5711951 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature