A ¹³C-detected ¹⁵N double-quantum NMR experiment to probe arginine side-chain guanidinium ¹⁵N^η chemical shifts.

ABSTRACT: Arginine side-chains are often key for enzyme catalysis, protein-ligand and protein-protein interactions. The importance of arginine stems from the ability of the terminal guanidinium group to form many key interactions, such as hydrogen bonds and salt bridges, as well as its perpetual positive charge. We present here an arginine ¹³C^ζ-detected NMR experiment in which a double-quantum coherence involving the two ¹⁵N^η nuclei is evolved during the indirect chemical shift evolution period. As the precession frequency of the double-quantum coherence is insensitive to exchange of the two ¹⁵N^η; this new approach is shown to eliminate the previously deleterious line broadenings of ¹⁵N^η resonances caused by the

SUBMITTER: Mackenzie HW 

PROVIDER: S-EPMC5711973 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature