GPI-anchored proteins are confined in subdiffraction clusters at the apical surface of polarized epithelial cells.

ABSTRACT: Spatio-temporal compartmentalization of membrane proteins is critical for the regulation of diverse vital functions in eukaryotic cells. It was previously shown that, at the apical surface of polarized MDCK cells, glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are organized in small cholesterol-independent clusters of single GPI-AP species (homoclusters), which are required for the formation of larger cholesterol-dependent clusters formed by multiple GPI-AP species (heteroclusters). This clustered organization is crucial for the biological activities of GPI-APs; hence, understanding the spatio-temporal properties of their membrane organization is of fundamental importance. Here, by using direct stochastic optical reconstruction microscopy coupled to pair correlation analysi

SUBMITTER: Paladino S 

PROVIDER: S-EPMC5712066 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature