Chronic wounds alter the proteome profile in skin mucus of farmed gilthead seabream.

ABSTRACT:

Background

Skin and its mucus are known to be the first barrier of defence against any external stressors. In fish, skin wounds frequently appear as a result of intensive culture and also some diseases have skin ulcers as external clinical signs. However, there is no information about the changes produced by the wounds in the mucosae. In the present paper, we have studied the alterations in the proteome map of skin mucus of gilthead seabream during healing of experimentally produced chronic wounds by 2-DE followed by LC-MS/MS. The corresponding gene expression changes of some identified skin proteins were also investigated through qPCR.

Results

Our study has successfully identified 21 differentially expressed proteins involved in immunity and stress processes as well as othe

SUBMITTER: Cordero H 

PROVIDER: S-EPMC5712093 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature