TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golgi.

ABSTRACT: The specificity of membrane traffic involves tethers at destination organelles that selectively capture incoming transport vesicles to allow SNAREs on opposing membranes to then assemble and drive fusion. Tethers include both protein complexes and long coiled-coil proteins, although how they contribute to specificity remains unclear. The golgin coiled-coil proteins at the Golgi apparatus capture vesicles from different origins, but the vesicle-specific molecular cues that they recognize are unknown. Vesicle tethering is typically a transient process and therefore is challenging to interrogate in vivo. Thus, we have used a system in which an ectopic golgin causes vesicles to accumulate in a tethered state. By applying proximity biotinylation to the golgin-captured vesicles, we identify TBC1

SUBMITTER: Shin JJH 

PROVIDER: S-EPMC5712222 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature