Global Protease Activity Profiling Provides Differential Diagnosis of Pancreatic Cysts.

ABSTRACT: Purpose: Pancreatic cysts are estimated to be present in 2%-3% of the adult population. Unfortunately, current diagnostics do not accurately distinguish benign cysts from those that can progress into invasive cancer. Misregulated pericellular proteolysis is a hallmark of malignancy, and therefore, we used a global approach to discover protease activities that differentiate benign nonmucinous cysts from premalignant mucinous cysts.Experimental Design: We employed an unbiased and global protease profiling approach to discover protease activities in 23 cyst fluid samples. The distinguishing activities of select proteases was confirmed in 110 samples using specific fluorogenic substrates and required less than 5 μL of cyst fluid.Results: We determined that the activities o

SUBMITTER: Ivry SL 

PROVIDER: S-EPMC5712228 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature