Analysis of NSP4 Gene and Its Association with Genotyping of Rotavirus Group A in Stool Samples

ABSTRACT:

Background

Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and E2 have been shown to be the most common genotypes in human.

Methods

The gene and protein sequence of NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin and other domains. P and G typings of RV samples were carried out by WHO primers using a semi-multiplex PCR method. Non-typeable RV samples were amplified by conserved primers and sequenced.

Results

In viroporin and enterotoxin, conserved

SUBMITTER: Teimoori A 

PROVIDER: S-EPMC5712384 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature