Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa.

ABSTRACT: Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments u

SUBMITTER: Dangol S 

PROVIDER: S-EPMC5712512 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature