Identification, Expression, and Functional Analysis of the Fructokinase Gene Family in Cassava.

ABSTRACT: Fructokinase (FRK) proteins play important roles in catalyzing fructose phosphorylation and participate in the carbohydrate metabolism of storage organs in plants. To investigate the roles of FRKs in cassava tuber root development, seven FRK genes (MeFRK1-7) were identified, and MeFRK1-6 were isolated. Phylogenetic analysis revealed that the MeFRK family genes can be divided into α (MeFRK1, 2, 6, 7) and β (MeFRK3, 4, 5) groups. All the MeFRK proteins have typical conserved regions and substrate binding residues similar to those of the FRKs. The overall predicted three-dimensional structures of MeFRK1-6 were similar, folding into a catalytic domain and a β-sheet ''lid" region, forming a substrate binding cleft, which contains many residues involved in the binding to fructose. The gene and t

SUBMITTER: Yao Y 

PROVIDER: S-EPMC5713366 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature