Doping of Green Fluorescent Protein into Superfluid Helium Droplets: Size and Velocity of Doped Droplets.

ABSTRACT: We report doping of green fluorescent protein from an electrospray ionization (ESI) source into superfluid helium droplets. From analyses of the time profiles of the doped droplets, we identify two distinct groups of droplets. The faster group has a smaller average size, on the order of 10⁶ helium atoms/droplet, and the slower group is much larger, by at least an order of magnitude. The relative populations of these two groups depend on the temperature of the droplet source: from 11 to 5 K, the signal intensity of the slower droplet group gradually increases, from near the detection limit to comparable to that of the faster group. We postulate that the smaller droplets are formed via condensation of gaseous helium upon expansion from the pulsed valve, while the larger droplets d

SUBMITTER: Alghamdi M 

PROVIDER: S-EPMC5713884 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature