An Ilomastat-CD Eye Drop Formulation to Treat Ocular Scarring.

ABSTRACT:

Purpose

The purpose of this study was to develop a topical matrix metalloproteinase inhibitor preparation for antiscarring therapy.

Methods

The broad spectrum matrix metalloproteinase inhibitor ilomastat was formulated using 2-hydroxypropyl-β-cyclodextrin in aqueous solution. In vitro activity of ilomastat-cyclodextrin (ilomastat-CD) was examined using fibroblasts seeded in collagen. Permeation of ilomastat-CD eye drop through pig eye conjunctiva was confirmed using Franz diffusion cells. Ilomastat-CD eye drop was applied to rabbit eyes in vivo, and the distribution of ilomastat in ocular tissues and fluids was determined by liquid chromatography-mass spectroscopy.

Results

The aqueous solubility of ilomastat-CD was ∼1000 μg/mL in water and 1400 μg/mL in PBS (pH 7.4),

SUBMITTER: Mohamed-Ahmed AHA 

PROVIDER: S-EPMC5713897 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature