Incomplete MyoD-induced transdifferentiation is associated with chromatin remodeling deficiencies.

ABSTRACT: Our current understanding of cellular transdifferentiation systems is limited. It is oftentimes unknown, at a genome-wide scale, how much transdifferentiated cells differ quantitatively from both the starting cells and the target cells. Focusing on transdifferentiation of primary human skin fibroblasts by forced expression of myogenic transcription factor MyoD, we performed quantitative analyses of gene expression and chromatin accessibility profiles of transdifferentiated cells compared to fibroblasts and myoblasts. In this system, we find that while many of the early muscle marker genes are reprogrammed, global gene expression and accessibility changes are still incomplete when compared to myoblasts. In addition, we find evidence of epigenetic memory in the transdifferentiated cells, wit

SUBMITTER: Manandhar D 

PROVIDER: S-EPMC5714206 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature