CuRRBS: simple and robust evaluation of enzyme combinations for reduced representation approaches.

ABSTRACT: DNA methylation is an important epigenetic modification in many species that is critical for development, and implicated in ageing and many complex diseases, such as cancer. Many cost-effective genome-wide analyses of DNA modifications rely on restriction enzymes capable of digesting genomic DNA at defined sequence motifs. There are hundreds of restriction enzyme families but few are used to date, because no tool is available for the systematic evaluation of restriction enzyme combinations that can enrich for certain sites of interest in a genome. Herein, we present customised Reduced Representation Bisulfite Sequencing (cuRRBS), a novel and easy-to-use computational method that solves this problem. By computing the optimal enzymatic digestions and size selection steps required, cuRRBS gen

SUBMITTER: Martin-Herranz DE 

PROVIDER: S-EPMC5714207 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature