Critical Modulation of Hematopoietic Lineage Fate by Hepatic Leukemia Factor.

ABSTRACT: A gradual restriction in lineage potential of multipotent stem/progenitor cells is a hallmark of adult hematopoiesis, but the underlying molecular events governing these processes remain incompletely understood. Here, we identified robust expression of the leukemia-associated transcription factor hepatic leukemia factor (Hlf) in normal multipotent hematopoietic progenitors, which was rapidly downregulated upon differentiation. Interference with its normal downregulation revealed Hlf as a strong negative regulator of lymphoid development, while remaining compatible with myeloid fates. Reciprocally, we observed rapid lymphoid commitment upon reduced Hlf activity. The arising phenotypes resulted from Hlf binding to active enhancers of myeloid-competent cells, transcriptional induction of myel

SUBMITTER: Wahlestedt M 

PROVIDER: S-EPMC5714592 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature