Hetero-multivalent binding of cholera toxin subunit B with glycolipid mixtures.

ABSTRACT: GM₁ has generally been considered as the major receptor that binds to cholera toxin subunit B (CTB) due to its low dissociation constant. However, using a unique nanocube sensor technology, we have shown that CTB can also bind to other glycolipid receptors, fucosyl-GM₁ and GD₁b. Additionally, we have demonstrated that GM₂ can contribute to CTB binding if present in a glycolipid mixture with a strongly binding receptor (GM₁/fucosyl-GM₁/GD₁b). This hetero-multivalent binding result was unintuitive because the interaction between CTB and pure GM₂ is negligible. We hypothesized that the reduced dimensionality of CTB-GM₂ binding events is a major cause of the observed CTB binding enhancement. Once

SUBMITTER: Krishnan P 

PROVIDER: S-EPMC5714677 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature