Positive rheotaxis extended drop: a one-step procedure to select and recover sperm with mature chromatin for intracytoplasmic sperm injection.

ABSTRACT:

Purpose

The purpose of this study was to develop a novel one-step ICSI approach to select sperm with better chromatin maturity than the conventional method.

Methods

This was a pilot diagnostic study, which prospectively recruited men during a 6-month period in a University-affiliated infertility centre. Forty consecutive semen samples were provided for analysis. The positive rheotaxis extended drop (PRED) was set up creating a pressure and viscosity gradient. Each semen sample was divided into four aliquots: one aliquot for density gradient centrifugation (DGC), two aliquots for PRED (fresh semen (PRED-FS) and processed semen (PRED-DGC)), and one aliquot as the control (FS). In PRED, a mean of 200 spermatozoa were collected consecutively without selection from the outlet res

SUBMITTER: De Martin H 

PROVIDER: S-EPMC5714818 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature