Evaluating lipopolysaccharide-induced oxidative stress in bovine granulosa cells.

ABSTRACT:

Purpose

The purpose of this study was to evaluate the capacity of bovine granulosa cells to generate reactive oxygen intermediates in response to lipopolysaccharide. We hypothesized that granulosa cells increase reactive oxygen intermediates in response to Gram-negative lipopolysaccharide in a similar manner to immune cells.

Methods

Bovine peripheral blood mononuclear cells and granulosa cells were cultured in the presence of lipopolysaccharide. Oxidative stress was evaluated using the fluorescent marker dye CellROX, and oxidative stress-related genes were measured using real-time RT-PCR.

Results

As expected, peripheral blood mononuclear cells increased oxidative stress in response to lipopolysaccharide as measured by accumulation of the fluorescent marker dye CellROX

SUBMITTER: Bromfield JJ 

PROVIDER: S-EPMC5714823 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature