Colonic motor dysfunctions in a mouse model of high-fat diet-induced obesity: an involvement of A_2B adenosine receptors.

ABSTRACT: Adenosine A_2B receptors (A_2BR) regulate several enteric functions. However, their implication in the pathophysiology of intestinal dysmotility associated with high-fat diet (HFD)-induced obesity has not been elucidated. We investigated the expression of A_2BR in mouse colon and their role in the mechanisms underlying the development of enteric dysmotility associated with obesity. Wild-type C57BL/6J mice were fed with HFD (60% kcal from fat) or normocaloric diet (NCD; 18% kcal from fat) for 8 weeks. Colonic A_2BR localization was examined by immunofluorescence. The role of A_2BR in the control of colonic motility was examined in functional experiments on longitudinal muscle preparations (LMPs). In NCD mice, A_2BR were predominant

SUBMITTER: Antonioli L 

PROVIDER: S-EPMC5714840 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature