Functional Study of Cytochrome P450 Enzymes from the Brown Planthopper (Nilaparvata lugens Stal) to Analyze Its Adaptation to BPH-Resistant Rice.

ABSTRACT: Plant-insect interactions constitute a complex of system, whereby plants synthesize toxic compounds as the main defense strategy to combat herbivore assault, and insects deploy detoxification systems to cope with toxic plant compounds. Cytochrom P450s are among the main detoxification enzymes employed by insects to combat the chemical defenses of host plants. In this study, we used Nilaparvata lugens (BPH) to constitute an ideal system for studying plant-insect interactions. By feeding BPHs with artificial diets containing ethanol extracts, we show that biotype Y BPHs have a greater ability to metabolize exogenous substrates than biotype 1 BPHs. NlCPR knockdown inhibited the ability of BPHs to feed on YHY15. qRT-PCR was used to screen genes in the P450 family, and upregulatio

SUBMITTER: Peng L 

PROVIDER: S-EPMC5714877 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature