Odorant Receptor 51E2 Agonist β-ionone Regulates RPE Cell Migration and Proliferation.

ABSTRACT: The odorant receptor 51E2 (OR51E2), which is well-characterized in prostate cancer cells and epidermal pigment cells, was identified for the first time as the most highly expressed OR in human fetal and adult retinal pigment epithelial (RPE) cells. Immunofluorescence staining and Western blot analysis revealed OR51E2 localization throughout the cytosol and in the plasma membrane. Additionally, immunohistochemical staining of diverse layers of the eye showed that the expression of OR51E2 is restricted to the pigment cells of the RPE and choroid. The results of Ca²⁺-imaging experiments demonstrate that activation of OR51E2 triggers a Ca²⁺ dependent signal pathway in RPE cells. Downstream signaling of OR51E2 involves the activation of adenylyl cyclase, ERK1/2 and AKT. Th

SUBMITTER: Jovancevic N 

PROVIDER: S-EPMC5714887 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature