Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology.

ABSTRACT: Organotypic co-cultures bridge the gap between standard two-dimensional culture and mouse models. Such assays increase the fidelity of pre-clinical studies, to better inform lead compound development and address the increasing attrition rates of lead compounds within the pharmaceutical industry, which are often a result of screening in less faithful two-dimensional models. Using large-scale acid-extraction techniques, we demonstrate a step-by-step process to isolate collagen I from commercially available animal byproducts. Using the well-established rat tail tendon collagen as a benchmark, we apply our novel kangaroo tail tendon collagen as an alternative collagen source for our screening-ready three-dimensional organotypic co-culture platform. Both collagen sources showed equal applicabil

SUBMITTER: Conway JRW 

PROVIDER: S-EPMC5715059 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature