Remote light-controlled intracellular target recognition by photochromic fluorescent glycoprobes.

ABSTRACT: Development of powerful fluorescence imaging probes and techniques sets the basis for the spatiotemporal tracking of cells at different physiological and pathological stages. While current imaging approaches rely on passive probe-analyte interactions, here we develop photochromic fluorescent glycoprobes capable of remote light-controlled intracellular target recognition. Conjugation between a fluorophore and spiropyran produces the photochromic probe, which is subsequently equipped with a glycoligand "antenna" to actively localize a target cell expressing a selective receptor. We demonstrate that the amphiphilic glycoprobes that form micelles in water can selectively enter the target cell to operate photochromic cycling as controlled by alternate UV/Vis irradiations. We further show that r

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5715093 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature