Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer's disease diagnostics.

ABSTRACT: Gene expression profiles reflect the biologically diverse activities of cells under specific cell environments. Using the transcriptional response of cultured cells to blood composition, we developed a litmus gene assay to discriminate blood samples reflecting different sample qualities or disease conditions. This cell-based litmus gene assay identified six genes (CCL20, CEMIP, IL1B, IL8, PRG2, PTGS2) as potential biomarkers of plasma quality control and the SPC25 gene as a diagnostic biomarker of Alzheimer's disease (AD). In addition, the SPC25 gene expression level was significantly increased in the cell-based assay using serum samples from patients with mild cognitive impairment (MCI). In conclusion, we demonstrated the effectiveness and potential of a litmus gene assay to detect the or

SUBMITTER: Shim SM 

PROVIDER: S-EPMC5715155 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature