Stable Europium(III) Complexes with Short Linkers for Site-Specific Labeling of Biomolecules.

ABSTRACT: In this study, two new terpyridine-based Eu^III complexes were synthesized, the structures of which were optimized for luminescence resonance energy-transfer (LRET) experiments. The complexes showed high quantum yields (32 %); a single long lifetime (1.25 ms), which was not influenced by coupling to protein; very high stability in the presence of chelators such as ethylenediamine-N,N,N',N'-tetraacetate and ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid; and no interaction with cofactors such as adenosine triphosphate and guanosine triphosphate. A special feature is the short length of the linker between the Eu^III ion and the maleimide or hydrazide function, which allows for site-specific couplin

SUBMITTER: Faschinger F 

PROVIDER: S-EPMC5715356 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature