Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development.

ABSTRACT:

Background

The differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at single-cell resolution, and a priori knowledge of limited biomarkers could restrict trajectory tracking.

Results

We employed marker-free single-cell RNA-Seq to characterize comprehensive transcriptional profiles of 507 cells randomly selected from seven stages between embryonic day 11.5 and postnatal day 2.5 during mouse liver development, and also 52 Epcam-positive cholangiocytes from postnatal day 3.25 mouse livers. LSPCs in developing mouse livers were identified via marker-free transcriptomic profiling. Single-cell resolution dynamic developmental trajectories of LSPCs exhibited contiguous but discrete genetic control through transcription f

SUBMITTER: Su X 

PROVIDER: S-EPMC5715535 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature