Diffusion of Soluble Aggregates of THIOMABs and Bispecific Antibodies in Serum.

ABSTRACT: Submicrometer aggregates are frequently present at low levels in antibody-based therapeutics. Although intuition suggests that the fraction of the aggregate or the size of the aggregate present might correlate with deleterious clinical properties or formulation difficulties, it has been challenging to demonstrate which aggregate states, if any, trigger specific biological effects. One source of uncertainty about the putative linkage between aggregation and safety or efficacy lies in the likelihood that noncovalent aggregation differs in ideal buffers versus in serum and biological tissues; self-association or association with other proteins may vary widely with environment. Therefore, methods for monitoring aggregation and aggregate behavior in biologically relevant matrices could provide

SUBMITTER: Goulet DR 

PROVIDER: S-EPMC5715667 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature