Lineage-specific dynamic and pre-established enhancer-promoter contacts cooperate in terminal differentiation.

ABSTRACT: Chromosome conformation is an important feature of metazoan gene regulation; however, enhancer-promoter contact remodeling during cellular differentiation remains poorly understood. To address this, genome-wide promoter capture Hi-C (CHi-C) was performed during epidermal differentiation. Two classes of enhancer-promoter contacts associated with differentiation-induced genes were identified. The first class ('gained') increased in contact strength during differentiation in concert with enhancer acquisition of the H3K27ac activation mark. The second class ('stable') were pre-established in undifferentiated cells, with enhancers constitutively marked by H3K27ac. The stable class was associated with the canonical conformation regulator cohesin, whereas the gained class was not, implying distin

SUBMITTER: Rubin AJ 

PROVIDER: S-EPMC5715812 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature