Human podocytes express functional thermosensitive TRPV channels.

ABSTRACT:

Background and purpose

Heat-sensitive transient receptor potential vanilloid (TRPV) channels are expressed in various epithelial tissues regulating, among else, barrier functions. Their expression is well established in the distal nephron; however, we have no data about their presence in podocytes. As podocytes are indispensable in the formation of the glomerular filtration barrier, we investigated the presence and function of Ca²⁺ -permeable TRPV1-4 channels in human podocyte cultures.

Experimental approach

Expression of TRPV1-4 channels was investigated at protein (immunocytochemistry, Western blot) and mRNA (Q-PCR) level in a conditionally immortalized human podocyte cell line. Channel function was assessed by measuring intracellular Ca²⁺ concentrati

SUBMITTER: Ambrus L 

PROVIDER: S-EPMC5715976 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature