A structural basis for the antibiotic resistance conferred by an N1-methylation of A1408 in 16S rRNA.

ABSTRACT: The aminoglycoside resistance conferred by an N1-methylation of A1408 in 16S rRNA by a novel plasmid-mediated methyltransferase NpmA can be a future health threat. In the present study, we have determined crystal structures of the bacterial ribosomal decoding A site with an A1408m1A antibiotic-resistance mutation both in the presence and absence of aminoglycosides. G418 and paromomycin both possessing a 6'-OH group specifically bind to the mutant A site and disturb its function as a molecular switch in the decoding process. On the other hand, binding of gentamicin with a 6'-NH3+ group to the mutant A site could not be observed in the present crystal structure. These observations agree with the minimum inhibitory concentration of aminoglycosides against Escherichia coli. In addition, one of

SUBMITTER: Kanazawa H 

PROVIDER: S-EPMC5716097 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature