Ritornello: high fidelity control-free chromatin immunoprecipitation peak calling.

ABSTRACT: With the advent of next generation high-throughput DNA sequencing technologies, omics experiments have become the mainstay for studying diverse biological effects on a genome wide scale. Chromatin immunoprecipitation (ChIP-seq) is the omics technique that enables genome wide localization of transcription factor (TF) binding or epigenetic modification events. Since the inception of ChIP-seq in 2007, many methods have been developed to infer ChIP-target binding loci from the resultant reads after mapping them to a reference genome. However, interpreting these data has proven challenging, and as such these algorithms have several shortcomings, including susceptibility to false positives due to artifactual peaks, poor localization of binding sites and the requirement for a total DNA input cont

SUBMITTER: Stanton KP 

PROVIDER: S-EPMC5716106 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature