Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.

ABSTRACT: Like tissues, single cells are subjected to continual stresses and damage. As such, cells have a robust wound repair mechanism comprised of dynamic membrane resealing and cortical cytoskeletal remodeling. One group of proteins, the Rho family of small guanosine triphosphatases (GTPases), is critical for this actin and myosin cytoskeletal response in which they form distinct dynamic spatial and temporal patterns/arrays surrounding the wound. A key mechanistic question, then, is how these GTPase arrays are formed. Here, we show that in the Drosophila melanogaster cell wound repair model Rho GTPase arrays form in response to prepatterning by Rho guanine nucleotide exchange factors (RhoGEFs), a family of proteins involved in the activation of small GTPases. Furthermore, we show that Ann

SUBMITTER: Nakamura M 

PROVIDER: S-EPMC5716286 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature