Development of a Pipeline for Exploratory Metabolic Profiling of Infant Urine.

ABSTRACT: Numerous metabolic profiling pipelines have been developed to characterize the composition of human biofluids and tissues, the vast majority of these being for studies in adults. To accommodate limited sample volume and to take into account the compositional differences between adult and infant biofluids, we developed and optimized sample handling and analytical procedures for studying urine from newborns. A robust pipeline for metabolic profiling using NMR spectroscopy was established, encompassing sample collection, preparation, spectroscopic measurement, and computational analysis. Longitudinal samples were collected from five infants from birth until 14 months of age. Methods of extraction and effects of freezing and sample dilution were assessed, and urinary contaminants from breakdow

SUBMITTER: Jackson F 

PROVIDER: S-EPMC5716460 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature