An NGS-based approach for the identification of sex-specific markers in snakehead (Channa argus).

ABSTRACT: We described a next generation sequencing (NGS)-based approach to identify sex-specific markers and subsequently determine whether a species has male or female heterogamety. To test the accuracy of this technique, we examined the snakehead (Channa argus), which is economically important freshwater fish in China. Males grow faster than females, and there is significant interest in developing methods to skew breeding towards all-males to increase biomass yields. NGS was conducted on DNAs of individual female and male, the male reads were spitted into 60 bp K-mers and aligned to the female reference genome assembled by female reads, unaligned male K-mers-60 were kept in next filter process. Meanwhile, DNA sample of 48 females was pooled and sequenced, this data was further used to filt

SUBMITTER: Ou M 

PROVIDER: S-EPMC5716763 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature