Using Click-Chemistry for Visualizing in Situ Changes of Translational Activity in Planktonic Marine Bacteria.

ABSTRACT: A major challenge in microbial ecology is linking diversity and function to determine which microbes are actively contributing to processes occurring in situ. Bioorthogonal non-canonical amino acid tagging (BONCAT) is a promising technique for detecting and quantifying translationally active bacteria in the environment. This technique consists of incubating a bacterial sample with an analog of methionine and using click-chemistry to identify the cells that have incorporated the substrate. Here, we established an optimized protocol for the visualization of protein-synthesizing cells in oligotrophic waters that can be coupled with taxonomic identification using Catalyzed Reporter Deposition Fluorescent in Situ Hybridization. We also evaluated the use of this technique to track shifts in tran

SUBMITTER: Leizeaga A 

PROVIDER: S-EPMC5717025 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature