Effects of 1,25 and 24,25 Vitamin D on Corneal Epithelial Proliferation, Migration and Vitamin D Metabolizing and Catabolizing Enzymes.

ABSTRACT: This study investigated the effects of 1,25(OH)₂D3 and 24R,25(OH)₂D3 on corneal epithelial cell proliferation, migration, and on the vitamin D activating enzyme CYP27B1 (produces 1,25(OH)₂D3) and inactivating enzyme CYP24A1 (produces 24R,25(OH)₂D3). The role of the vitamin D receptor (VDR) was also examined. In VDR wildtype mouse corneal epithelial cells (WT), 1,25(OH)₂D3 increased CYP24A1 protein expression and decreased CYP27B1 expression. In VDR knockout mouse epithelial cells (KO), 1,25(OH)₂D3 increased CYP24A1 and CYP27B1 protein expression. 1,25(OH)₂D3 did not affect WT cell proliferation, but did stimulate VDR KO cell proliferation. In a human corneal epithelial cell line (HCEC), 1,25(OH)₂D3 incr

SUBMITTER: Lu X 

PROVIDER: S-EPMC5717139 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature