Murein Hydrolase LytF of Streptococcus sanguinis and the Ecological Consequences of Competence Development.

ABSTRACT: The overall health of the oral cavity is dependent on proper homeostasis between health-associated bacterial colonizers and bacteria known to promote dental caries. Streptococcus sanguinis is a health-associated commensal organism, a known early colonizer of the acquired tooth pellicle, and is naturally competent. We have shown that LytF, a competence-controlled murein hydrolase, is capable of inducing the release of extracellular DNA (eDNA) from oral bacteria. Precipitated LytF and purified LytF were used as treatments against planktonic cultures and biofilms. Larger amounts of eDNA were released from cultures treated with protein samples containing LytF. Additionally, LytF could affect biofilm formation and cellular morphology. Biofilm formation was significantly decreased in the lytF-co

SUBMITTER: Cullin N 

PROVIDER: S-EPMC5717204 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature