High-resolution profiling of the gut microbiome reveals the extent of Clostridium difficile burden.

ABSTRACT: Microbiome profiling through 16S rRNA gene sequence analysis has proven to be a useful research tool in the study of C. difficile infection (CDI); however, CDI microbiome studies typically report results at the genus level or higher, thus precluding identification of this pathogen relative to other members of the gut microbiota. Accurate identification of C. difficile relative to the overall gut microbiome may be useful in assessments of colonization in research studies or as a prognostic indicator for patients with CDI. To investigate the burden of C. difficile at the species level relative to the overall gut microbiome, we applied a high-resolution method for 16S rRNA sequence assignment to previously published gut microbiome studies of CDI and other patient populati

SUBMITTER: Daquigan N 

PROVIDER: S-EPMC5717231 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature